Potency values were generated using wells treated with a broad concentration range of LNA oligomer, from which the resulting normalized Tau/Tuj-1 and Tuj-1 values were analyzed compared to normalized values from saline control samples.

Analysis was done using non-linear regression with top and bottom values set at fixed values of 100% and 0%, respectively, where 100% inhibition represents a complete reduction of signal compared to the control sample (FIG. 3). For qPCR, data were analyzed using a one-way ANOVA with a Dunnett's multiple comparison test to compare saline- and LNA oligomer-treated groups.
The cultures were then subject to immunocytochemistry and imaging to measure the inhibition. Nuclei counts from each well were also acquired as an alternative measure of toxicity associated with LNA oligomer treatment. The platform location remained the same throughout training whereas the drop location varied randomly between the four daily trials as well as across the 4 days of training.
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